Depletion of nuclear LINE1 RNA in mouse ESCs and embryos

Written By Santos Lab
 
1-s2.0-S2666166721004330-fx1_lrg.jpg
 

Authors: Percharde M, Lin CJ, Ramalho-Santos M.

STAR Protocols, Volume 2, Issue 3, 2021, 100726, ISSN 2666-1667.

Abstract: LINE1 is the most active and abundant family of retrotransposons; it is implicated in a number of pathologies, as well as in early embryo development. We present a protocol to specifically knockdown LINE1 in mouse embryonic stem cells and embryos, including details for the nucleofection and zygote microinjection of LINE antisense oligos, followed by RNA FISH validation. This protocol can be used in development, as well as other cell types where LINE1 is believed to be expressed.


Santos Lab
Previous

Regulation, functions and transmission of bivalent chromatin during mammalian development
Next

Chd1 regulates repair of promoter-proximal DNA breaks to sustain hypertranscription in embryonic stem cells